Available versus digestible amino acids – new stable isotope methods
نویسندگان
چکیده
منابع مشابه
Stable-isotope labeling by amino acids in cell culture (SILAC)
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For a dietary amino acid to be retained in tissue protein in an animal, the amino acid needs to be ingested by the animal and absorbed from the intestinal tract. While the absorption of amino acids is a concept that can easily be defined, it is difficult to measure. Instead, the digestibility of amino acids is measured. Digestibility is defined as the difference between the amount of a certain ...
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Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana has led to very limited use of SILAC in plant systems. We present a method allowing, for the first...
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ژورنال
عنوان ژورنال: British Journal of Nutrition
سال: 2012
ISSN: 0007-1145,1475-2662
DOI: 10.1017/s0007114512002498